The mixture of kresoxim-methyl and boscalid, an excellent alternative controlling grey mould caused by Botrytis cinerea | Baseline sensitivity; boscalid; Botrytis cinerea; kresoxim-methyl; sensitivity shift; Qo centre inhibitors (QoIs) | Grey mould, caused by the fungus Botrytis cinerea, is one of the most destructive diseases in greenhouses for which serious fungicide resistance has developed. Between 2003 and 2005, 213 isolates of B. cinerea from two geographical regions were characterised for baseline sensitivity to kresoxim-methyl. In the absence of salicylhydroxamic acid (SHAM), the mean 50% effective concentration (EC50) values were 6.67 0.61 (mean SD) and 0.37 0.10 mg L (1) during growth and germination, respectively. In the presence of 100 mg L (1) SHAM, baseline sensitivities were distributed as unimodal curves with mean EC50 values of 2.38 0.21 and 0.28 0.09 mg L (-1) for inhibiting growth and inhibiting germination, respectively. The mixture of kresoxim-methyl and boscalid showed good control efficacy against strawberry grey mould disease. After the mixture was extensively used on strawberry for 2 years, 50 isolates were collected and determined for their sensitivity to kresoxim-methyl and boscalid, respectively. The mean EC50 of germination inhibition by boscalid was 0.39 0.08 mg L (1). The mean EC50 of germination inhibition by kresoxim-methyl was 0.26 0.07 mg L-1 in the presence of 100 mg L (1) SHAM. Sensitivities of B. cinerea to both kresoxim-methyl and boscalid did not show any significant decrease. These results suggest that their mixture is a satisfactory alternative candidate for management of grey mould disease in greenhouses.

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