Method summary

Acetonitrile + water as the mobile phase, the samples were dissolved in mobile phase, use as filler in C18 stainless steel column and UV detector (255nm), the sample of azoxystrobin reversed-phase high-performance liquid chromatography, external standard quantification.

Reagents and solutions

Acetonitrile: HPLC grade water: freshly distilled twice distilled water

Mobile phase: ψ (acetonitrile: water) = 5: 3, through membrane filtration, and degassing azoxystrobin Sample: A known mass fraction ≥99.0%

Instrument

High performance liquid chromatography: a variable wavelength UV detector chromatographic data processor

Column: 250mm × 4.6mm (id) stainless steel column, filled with filler C18,5μm Filter: 0.45um membrane pore size of about micro-injector: 100μL quantitative injection tube: 5μL ultrasonic cleaner.

HPLC operating conditions

Flow rate: 1.0mL / min

Column temperature: room temperature (temperature change should not exceed 2 ℃) detection wavelength: 255nm Injection volume: 5μL

Retention time: azoxystrobin 5.5min